LOCUS pDestTol2pA2__39 5877 bp DNA circular 28-FEB-2025 COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 1097..1221 /note="attR4" /locus_tag="attR4" /label="attR4" /ApEinfo_label="attR4" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_recomb 2676..2800 /note="attR3" /locus_tag="attR3" /label="attR3" /ApEinfo_label="attR3" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind complement(2807..2824) /note="M13(-20)" /locus_tag="M13(-20)" /label="M13(-20)" /ApEinfo_label="M13(-20)" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" gene complement(4896..5753) /note="Amp" /locus_tag="Amp" /label="Amp" /ApEinfo_label="Amp" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" gene 1908..2567 /note="CAM" /locus_tag="CAM" /label="CAM" /ApEinfo_label="CAM" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_recomb 703..1049 /note="Tol2" /locus_tag="Tol2" /label="Tol2" /ApEinfo_label="Tol2" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_recomb 3096..3430 /note="Tol2, Rt arm" /locus_tag="Tol2, Rt arm" /label="Tol2, Rt arm" /ApEinfo_label="Tol2, Rt arm" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS 1261..1566 /note="ccdB" /locus_tag="ccdB" /label="ccdB" /ApEinfo_label="ccdB" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter complement(3694..3710) /note="T3" /locus_tag="T3" /label="T3" /ApEinfo_label="T3" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind complement(3691..3710) /locus_tag="T3(1)" /label="T3(1)" /ApEinfo_label="T3" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind complement(2808..2825) /locus_tag="M13-fwd" /label="M13-fwd" /ApEinfo_label="M13-fwd" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind 1061..1081 /locus_tag="M13-rev" /label="M13-rev" /ApEinfo_label="M13-rev" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind complement(3728..3748) /locus_tag="M13-rev(1)" /label="M13-rev(1)" /ApEinfo_label="M13-rev" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" rep_origin complement(4116..4798) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_binding complement(3754..3776) /locus_tag="LacO" /label="LacO" /ApEinfo_label="LacO" /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS complement(4896..5555) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS complement(1911..2567) /locus_tag="CmR" /label="CmR" /ApEinfo_label="CmR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" polyA_signal 2838..3029 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA 61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA 121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG 181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA 241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG 301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA 361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA 421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC 481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT 541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT 601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT 661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA 721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA 781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA 841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC 901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC 961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA 1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT 1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGAAC GAGAAACGTA AAATGATATA 1141 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA 1201 CAACATATCC AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT 1261 TTATATTCCC CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG 1321 ATCAGCCACT TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT 1381 GCGCCAGCTT TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGGGA CTTTATCTGA 1441 CAGCAGACGT GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC 1501 ACTCTGTACA TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA 1561 CTGCATTTCA CCAGCCCCTG TTCTCGTCGG CAAAGGAGCC GTTCATTTCA ATAAACCGGG 1621 CGACCTCAGC CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG 1681 CTTCATTCTG CATGGTTGTG CTTACCGAAC CGGAGATATT GACATCATAT ATGCCTTGAG 1741 CAACTGATAG CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT 1801 TTTGACATAC TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC 1861 AAATACGCAT ACTGTTATCT GGCTTTTAGT AAGCCGGATC CTCTAGATTA CGCCCCGCCC 1921 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 1981 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 2041 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 2101 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 2161 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 2221 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 2281 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 2341 GCCATACGGA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 2401 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 2461 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 2521 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA 2581 GCTCCTGAAA ATCTCGACGG ATCCTAACTC AAAATCCACA CATTATACGA GCCGGAAGCA 2641 TAAAGTGTAA AGCCTGGGGG TGCCTAATGC GGCCGCCATA GTGACTGGAT ATGTTGTGTT 2701 TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA 2761 TCATTTTACG TTTCTCGTTC AACTTTATTA TACATAGTTG ATAATTCACT GGCCGTCGTT 2821 TTACATCGAT GATGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC 2881 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT 2941 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA 3001 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 3061 GGCTGATTAT GATCCTCTAG ATCAGATCTG CGAAGATACG GCCACGGGTG CTCTTGATCC 3121 TGTGGCTGAT TTTGGACTGT GCTGCTCGCA GCTGCTGATG AATCACATAC TTCCTCCATT 3181 TTCTTCCACT GATTGACTGT TATAATTTCC CTAATTTCCA GGTCAAGGTG CTGTGCATTG 3241 TGGTAATAGA TGTGACATGA CGTCACTTCC AAAGGACCAA TGAACATGTC TGACCAATTT 3301 CATATAATGT GAAAACGATT TTCATAGGCA GAATAAATAA CATTTAAATT AAACTGGGCA 3361 TCAGCGCAAT TCAATTGGTT TGGTAATAGC AAGGGAAAAT AGAATGAAGT GATCTCCAAA 3421 AAATAAGTAC TTTTTGACTG TAAATAAAAT TGTAAGGAGT AAAAAGTACT TTTTTTTCTA 3481 AAAAAATGTA ATTAAGTAAA AGTAAAAGTA TTGATTTTTA ATTGTACTCA AGTAAAGTAA 3541 AAATCCCCAA AAATAATACT TAAGTACAGT AATCAAGTAA AATTACTCAA GTACTTTACA 3601 CCTCTGGTTC TTGACCCCCT ACCTTCAGCA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG 3661 CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG 3721 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 3781 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 3841 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 3901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 3961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 4021 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4081 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4141 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 4201 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 4261 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 4321 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4381 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 4441 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 4501 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 4561 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 4621 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 4681 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 4741 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 4801 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 4861 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 4921 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 4981 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 5041 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 5101 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 5161 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 5221 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 5281 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 5341 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 5401 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 5461 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 5521 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 5581 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 5641 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 5701 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 5761 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 5821 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTG //